A reverse phase high performance liquid chromatography (RP-HPLC) method for the simultaneous estimation of Metoprolol succinate and Clopidogrel bisuphate in marketed formulation is developed. The determination was carried out on a Hibar 250-4.6 RP18 (5um) column using a mobile phase of Methanol: Water: Acetonitrile (70:20:10) pH-3.4 with ortho phosphoric acid. The flow rate was 1.0 ml/min with detection at 222 nm.The retention time for Metoprolol succinate 2.1 min and Clopidogrel bisulphate 7.0 min. Metoprolol succinate and Clopidogrel bisulphate showed a linear response in the concentration range of 5-40µg/ml and 7.5-60µg/ml respectively.The correlation co-efficient (r2) 0.997 for Metoprolol succinate and 0.999 for Clopidogrel bisulphate.The results of analysis have been validated statistically and by recovery studies.The percentage recoveries obtained for Metoprolol succinate and Clopidogrel bisulphate ranges from 99.05-101.28%.
Loading....